Journal: bioRxiv

Article Title: iRhom2 serves as a facilitator in obesity by enhancing adipose inflammation and insulin resistance

doi: 10.1101/600460

Figure Lengend Snippet: iRhom2 contributes to adipose inflammatory responses in of obese mice. (A) Representative immunoblot bands for expression of inflammation related signaling including TNFR1/2, phosphorylated IκBα, NF-κB, TAK1 and TNF-α. qPCR analysis detection for mRNA expression of genes responsible for (B) lipid metabolisms and (C) inflammatory cytokines and chemokines in HFD-fed WT mice and iRhom2 mice, n =6. Representative western blotting bands for expression of inflammatory signaling in (D) adipocytes and (E) F4/80+ macrophages isolated from iWAT of HFD-fed WT mice and iRhom2 mice. (F) Western blotting analysis for inflammatory signaling in iWAT of HFD-fed AAV-iRhom2 over-activated mice and corresponding mice. mRNA expression of genes responsible for (G) lipid metabolisms and (H) inflammatory cytokines and chemokines in HFD-fed AAV-control mice and AAV-iRhom2 over-activated mice, n =6. Western blotting for expression of inflammation indicators in (D) adipocytes and (E) F4/80+ macrophages from iWAT of AAV-control mice and AAV-iRhom2 over-activated mice. Data were expressed as mean ± SEM, * p < 0.05.

Article Snippet: In brief, 2 × 10 SVF cells was re-suspended in 120 μl Hanks buffer (Solarbio, Beijing, China) and then stained with 15 μl mouse PE-conjugated F4/80 mAbs (Cat#: FAB5580P-100UG, R&D system), and co-incubated with 30 μl anti-PE magnetic beads for 20 min. Next, the washed cells were re-suspended in 1 ml Hanks buffer and then subjected to XS Separation columns (Miltenyl Biotec MACS).

Techniques: Western Blot, Expressing, Isolation

Journal: bioRxiv

Article Title: iRhom2 serves as a facilitator in obesity by enhancing adipose inflammation and insulin resistance

doi: 10.1101/600460

Figure Lengend Snippet: Myeloid cell-specific iRhom2 deletion down regulates insulin resistance and inflammation in obese mice. (A) Body weight was examined before and after the feeding period, n =15. (B) Body composition, n =10. (C) Glucose and (D) insulin tolerance test, n =15. Western blotting bands for expression of (E) insulin signaling from iWAT and (F) inflammation signaling of F4/80+ macrophages in iWAT. mRNA expression of genes responsible for (G) lipid metabolisms and (H) inflammatory cytokines and chemokines in HFD-fed WTBM→WT/HFD and KOBM→WT/HFD mice, n =6. Data were expressed as mean ± SEM, * p < 0.05.

Article Snippet: In brief, 2 × 10 SVF cells was re-suspended in 120 μl Hanks buffer (Solarbio, Beijing, China) and then stained with 15 μl mouse PE-conjugated F4/80 mAbs (Cat#: FAB5580P-100UG, R&D system), and co-incubated with 30 μl anti-PE magnetic beads for 20 min. Next, the washed cells were re-suspended in 1 ml Hanks buffer and then subjected to XS Separation columns (Miltenyl Biotec MACS).

Techniques: Western Blot, Expressing